In-vitro fertilization (IVF) is a procedure where eggs are harvested from a woman’s body, and fertilized in the IVF laboratory with her partner/donor sperm to create embryos that are later used for pregnancy.
- Diagnostic tests
During ovarian stimulation, injectable medications are used to recruit follicles/ eggs. Progress is monitored by blood tests and ultrasounds every 2-3 days. When the follicles appear ready a trigger medication is used to mature the eggs for egg retrieval 2 days later.
A. Standard Insemination:
Insemination through IVF refers to incubation of washed sperm with the harvested eggs in a petri dish to allow fertilization. 30,000 sperm are incubated per egg and fertilization is allowed to occur “naturally” in the dish.
B. Intracytoplasmic sperm injection (ICSI):
The rate of fertilization of mature eggs is typically 60-70% (i.e. 7/ 10 mature eggs fertilize) provided in appropriate cases. Assisted fertilization can be accomplished through
Fertilized eggs are cultured in the IVF Laboratory until transfer, biopsy or freezing. An intricate balance of gases and culture media choices optimize outcomes.
To date the standard method of embryo selection has been morphology. Embryo morphology is assessed and scored based on cleavage kinetics, cell numbers, extent of fragmentation and cytoplasmic anomalies. This criteria is for the purpose of selecting the “best” embryo(s) for transfer.
The goal is to transfer a blastocyst on day 5 of embryo development; however, the decision to do a day 3 versus day 5 transfer is individualized to the patient’s history, ovarian reserve, number of embryos and desire/ need for additional assisted reproductive techniques such as CCS/ PGS or PGD.
The number of embryos transferred depends a patient’s age and history. Genesis Fertility Centre is committed to healthy babies and healthy mothers. We are therefore a proponent of singleton pregnancies which have the best outcomes for both.
We have developed and use mathematical models to help predict how many embryos to transfer based on maternal age, history and embryo quality at the time of transfer to optimize singleton pregnancies and IVF success rates. These models have been presented at international meetings and received international funding.
Additional methods for embryo selection including Eeva™ and Comprehensive chromosomal screening /pre-implantation genetic screening (CCS/PGS) .
B. Embryo Freeze
- Tubal disease
- Severe male factor
- Diminished ovarian reserve or advanced egg age
- Long duration of unexplained infertility
- Pre-implantation genetic diagnoses or pre-implantation genetic screening/ comprehensive chromosomal screening
- Previous failed treatments such as insemination, tubal surgery